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| 3. |  | PASTORE, T. C. M.; BRAGA, J. W. B.; CORADIN, V. T. R.; MAGALHAES, W. L. E.; OKINO, E. Y. A.; CAMARGOS, J. A. A.; BOLZON DE MUÑIZ, G. I.; BRESSAN, O. A.; DAVRIEUX, F. Near infrared spectroscopy (NIRS) as a potential tool for monitoring trade of similar woods: discrimination of true mahogany, cedar, andiroba, and curupixa. Holzforschung, v. 65, p. 73-80, 2011.| Biblioteca(s): Embrapa Florestas. |
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| 4. | | PASTORE, T. C. M.; BRAGA, J. W. B.; CORADIN, V. T. R.; MAGALHAES, W. L. E.; OKINO, E. Y. A.; CAMARGOS, J. A. A.; BOLZON DE MUÑIZ, G. I.; DAVRIEUX, F.; BRESSAN, O. A. NIRS a potential tool for monitoring harvesting and trade of similar woods: a case study for discrimination of mahogany, cedar, and crabwood. In: CONGRESO FORESTAL MUNDIAL, 13., 2009, Buenos Aires. Desarrollo forestal: equilibrio vital. Argentina: FAO, 2009. Resumo.| Biblioteca(s): Embrapa Florestas. |
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Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
07/12/2012 |
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Data da última atualização: |
13/07/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
DROVAL, A. A.; BINNECK, E.; MARIN, S. R. R.; PAIÃO, F. G.; OBA, A.; NEPOMUCENO, A. L.; SHIMOKOMAKI, M. |
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Afiliação: |
ADRIANA A. DROVAL, UEL; ELISEU BINNECK, CNPSO; SILVANA REGINA ROCKENBACH MARIN, CNPSO; FERNANDA G. PAIÃO, UEL; A. OBA, UEL; ALEXANDRE LIMA NEPOMUCENO, SRI; MASSAMI SHIMOKOMAKI, UTFPR. |
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Título: |
A new single nucleotide polymorphism in the ryanodine gene of chicken skeletal muscle. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Genetics and Molecular Research, Ribeirão Preto, v. 11, n. 2, p. 821-829, 2012. |
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Idioma: |
Inglês |
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Conteúdo: |
Some genes affect meat quality in chickens. We looked for polymorphisms in the Gallus gallus α-RyR gene (homologous to RyR 1) that could be associated with PSE (pale, soft and exudative) meat. Because RyR genes are over 100,000 bp long and code for proteins with about 5000 amino acids, primers were designed to amplify a fragment of hotspot region 2, a region with a high density of mutations in other species. Total blood DNA was extracted from 50 birds, 25 that had PSE meat and 25 normal chickens. The DNA samples were amplified by PCR, cloned, sequenced, and used to identify single nucleotide polymorphisms (SNPs). The amplified fragment of α-RyR was 604 nucleotides in length; 181 nucleotides were similar to two exons from a hypothetical turkey cDNA sequence for α-RyR. A non-synonymous nucleotide substitution (G/A) was identified in at least one of the three sequenced clones obtained from nine animals, six PSE (HAL+) birds and three normal (HAL-) birds; they were heterozygous for this mutation. |
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Thesagro: |
Carne; Genética; Mutação; Qualidade. |
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Thesaurus NAL: |
Genetics; Meat quality; Mutation. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/71833/1/new.single.gmr.11.2.2012.pdf
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Marc: |
LEADER 01771naa a2200277 a 4500
001 1941757
005 2017-07-13
008 2012 bl uuuu u00u1 u #d
100 1 $aDROVAL, A. A.
245 $aA new single nucleotide polymorphism in the ryanodine gene of chicken skeletal muscle.
260 $c2012
520 $aSome genes affect meat quality in chickens. We looked for polymorphisms in the Gallus gallus α-RyR gene (homologous to RyR 1) that could be associated with PSE (pale, soft and exudative) meat. Because RyR genes are over 100,000 bp long and code for proteins with about 5000 amino acids, primers were designed to amplify a fragment of hotspot region 2, a region with a high density of mutations in other species. Total blood DNA was extracted from 50 birds, 25 that had PSE meat and 25 normal chickens. The DNA samples were amplified by PCR, cloned, sequenced, and used to identify single nucleotide polymorphisms (SNPs). The amplified fragment of α-RyR was 604 nucleotides in length; 181 nucleotides were similar to two exons from a hypothetical turkey cDNA sequence for α-RyR. A non-synonymous nucleotide substitution (G/A) was identified in at least one of the three sequenced clones obtained from nine animals, six PSE (HAL+) birds and three normal (HAL-) birds; they were heterozygous for this mutation.
650 $aGenetics
650 $aMeat quality
650 $aMutation
650 $aCarne
650 $aGenética
650 $aMutação
650 $aQualidade
700 1 $aBINNECK, E.
700 1 $aMARIN, S. R. R.
700 1 $aPAIÃO, F. G.
700 1 $aOBA, A.
700 1 $aNEPOMUCENO, A. L.
700 1 $aSHIMOKOMAKI, M.
773 $tGenetics and Molecular Research, Ribeirão Preto$gv. 11, n. 2, p. 821-829, 2012.
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Registro original: |
Embrapa Soja (CNPSO) |
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